Reverse Transcription:Article Title: Nucleotide resolution sequencing of N4-acetylcytidine in RNA
Article Snippet: TGIRT-III reverse transcription enzyme (INGEX) 5× TGIRT reaction buffer (user prepared with nuclease-free reagents, 2.25M NaCl, 100mM Tris-HCL pH7.5) RNasin plus, 40units/μL (Promega, N2611) Optimized dNTP mix [10mM dTTP, 10mM dCTP, 10mM dATP, 5mM dGTP] (prepared from 100mM dNTPs set, NEB N0446S) Phusion High-Fidelity PCR Kit (New England Biolabs, E0553S) Agarose LE, Molecular Biology Grade (Thomas Scientific, C996H59) 100bp DNA Ladder (New England Biolabs, N3231S) SYBR Safe DNA Gel Stain (Invitrogen, {"type":"entrez-protein","attrs":{"text":"S33102","term_id":"420481","term_text":"pir||S33102"}} S33102 ) UltraPure TBE Buffer, 10× (Invitrogen, 15581044) UV transilluminator (UVP MultiDoc-It Imager Benchtop UV Transilluminator) 6× DNA gel loading dye (NEB, B7021S) QIAquick Gel extraction kit (Qiagen, 28704) Thermocycler Agarose gel electrophoresis equipment.
Article Title: Nucleotide resolution sequencing of N4-acetylcytidine in RNA
Article Snippet: Materials TGIRT-III reverse transcription enzyme (INGEX) 5× TGIRT reaction buffer (user prepared with nuclease-free reagents, 2.25M NaCl, 100mM Tris-HCL pH7.5) RNasin plus, 40units/μL (Promega, N2611) Optimized dNTP mix [10mM dTTP, 10mM dCTP, 10mM dATP, 5mM dGTP] (prepared from 100mM dNTPs set, NEB N0446S) Phusion High-Fidelity PCR Kit (New England Biolabs, E0553S) Agarose LE, Molecular Biology Grade (Thomas Scientific, C996H59) 100bp DNA Ladder (New England Biolabs, N3231S) SYBR Safe DNA Gel Stain (Invitrogen, {"type":"entrez-protein","attrs":{"text":"S33102","term_id":"420481","term_text":"pir||S33102"}} S33102 ) UltraPure TBE Buffer, 10× (Invitrogen, 15581044) UV transilluminator (UVP MultiDoc-It Imager Benchtop UV Transilluminator) 6× DNA gel loading dye (NEB, B7021S) QIAquick Gel extraction kit (Qiagen, 28704) Thermocycler Agarose gel electrophoresis equipment.
Polymerase Chain Reaction:Article Title: Nucleotide resolution sequencing of N4-acetylcytidine in RNA
Article Snippet: TGIRT-III reverse transcription enzyme (INGEX) 5× TGIRT reaction buffer (user prepared with nuclease-free reagents, 2.25M NaCl, 100mM Tris-HCL pH7.5) RNasin plus, 40units/μL (Promega, N2611) Optimized dNTP mix [10mM dTTP, 10mM dCTP, 10mM dATP, 5mM dGTP] (prepared from 100mM dNTPs set, NEB N0446S) Phusion High-Fidelity PCR Kit (New England Biolabs, E0553S) Agarose LE, Molecular Biology Grade (Thomas Scientific, C996H59) 100bp DNA Ladder (New England Biolabs, N3231S) SYBR Safe DNA Gel Stain (Invitrogen, {"type":"entrez-protein","attrs":{"text":"S33102","term_id":"420481","term_text":"pir||S33102"}} S33102 ) UltraPure TBE Buffer, 10× (Invitrogen, 15581044) UV transilluminator (UVP MultiDoc-It Imager Benchtop UV Transilluminator) 6× DNA gel loading dye (NEB, B7021S) QIAquick Gel extraction kit (Qiagen, 28704) Thermocycler Agarose gel electrophoresis equipment.
Article Title: Nucleotide resolution sequencing of N4-acetylcytidine in RNA
Article Snippet: Materials TGIRT-III reverse transcription enzyme (INGEX) 5× TGIRT reaction buffer (user prepared with nuclease-free reagents, 2.25M NaCl, 100mM Tris-HCL pH7.5) RNasin plus, 40units/μL (Promega, N2611) Optimized dNTP mix [10mM dTTP, 10mM dCTP, 10mM dATP, 5mM dGTP] (prepared from 100mM dNTPs set, NEB N0446S) Phusion High-Fidelity PCR Kit (New England Biolabs, E0553S) Agarose LE, Molecular Biology Grade (Thomas Scientific, C996H59) 100bp DNA Ladder (New England Biolabs, N3231S) SYBR Safe DNA Gel Stain (Invitrogen, {"type":"entrez-protein","attrs":{"text":"S33102","term_id":"420481","term_text":"pir||S33102"}} S33102 ) UltraPure TBE Buffer, 10× (Invitrogen, 15581044) UV transilluminator (UVP MultiDoc-It Imager Benchtop UV Transilluminator) 6× DNA gel loading dye (NEB, B7021S) QIAquick Gel extraction kit (Qiagen, 28704) Thermocycler Agarose gel electrophoresis equipment.
Staining:Article Title: Nucleotide resolution sequencing of N4-acetylcytidine in RNA
Article Snippet: TGIRT-III reverse transcription enzyme (INGEX) 5× TGIRT reaction buffer (user prepared with nuclease-free reagents, 2.25M NaCl, 100mM Tris-HCL pH7.5) RNasin plus, 40units/μL (Promega, N2611) Optimized dNTP mix [10mM dTTP, 10mM dCTP, 10mM dATP, 5mM dGTP] (prepared from 100mM dNTPs set, NEB N0446S) Phusion High-Fidelity PCR Kit (New England Biolabs, E0553S) Agarose LE, Molecular Biology Grade (Thomas Scientific, C996H59) 100bp DNA Ladder (New England Biolabs, N3231S) SYBR Safe DNA Gel Stain (Invitrogen, {"type":"entrez-protein","attrs":{"text":"S33102","term_id":"420481","term_text":"pir||S33102"}} S33102 ) UltraPure TBE Buffer, 10× (Invitrogen, 15581044) UV transilluminator (UVP MultiDoc-It Imager Benchtop UV Transilluminator) 6× DNA gel loading dye (NEB, B7021S) QIAquick Gel extraction kit (Qiagen, 28704) Thermocycler Agarose gel electrophoresis equipment.
Article Title: Nucleotide resolution sequencing of N4-acetylcytidine in RNA
Article Snippet: Materials TGIRT-III reverse transcription enzyme (INGEX) 5× TGIRT reaction buffer (user prepared with nuclease-free reagents, 2.25M NaCl, 100mM Tris-HCL pH7.5) RNasin plus, 40units/μL (Promega, N2611) Optimized dNTP mix [10mM dTTP, 10mM dCTP, 10mM dATP, 5mM dGTP] (prepared from 100mM dNTPs set, NEB N0446S) Phusion High-Fidelity PCR Kit (New England Biolabs, E0553S) Agarose LE, Molecular Biology Grade (Thomas Scientific, C996H59) 100bp DNA Ladder (New England Biolabs, N3231S) SYBR Safe DNA Gel Stain (Invitrogen, {"type":"entrez-protein","attrs":{"text":"S33102","term_id":"420481","term_text":"pir||S33102"}} S33102 ) UltraPure TBE Buffer, 10× (Invitrogen, 15581044) UV transilluminator (UVP MultiDoc-It Imager Benchtop UV Transilluminator) 6× DNA gel loading dye (NEB, B7021S) QIAquick Gel extraction kit (Qiagen, 28704) Thermocycler Agarose gel electrophoresis equipment.
Gel Extraction:Article Title: Nucleotide resolution sequencing of N4-acetylcytidine in RNA
Article Snippet: TGIRT-III reverse transcription enzyme (INGEX) 5× TGIRT reaction buffer (user prepared with nuclease-free reagents, 2.25M NaCl, 100mM Tris-HCL pH7.5) RNasin plus, 40units/μL (Promega, N2611) Optimized dNTP mix [10mM dTTP, 10mM dCTP, 10mM dATP, 5mM dGTP] (prepared from 100mM dNTPs set, NEB N0446S) Phusion High-Fidelity PCR Kit (New England Biolabs, E0553S) Agarose LE, Molecular Biology Grade (Thomas Scientific, C996H59) 100bp DNA Ladder (New England Biolabs, N3231S) SYBR Safe DNA Gel Stain (Invitrogen, {"type":"entrez-protein","attrs":{"text":"S33102","term_id":"420481","term_text":"pir||S33102"}} S33102 ) UltraPure TBE Buffer, 10× (Invitrogen, 15581044) UV transilluminator (UVP MultiDoc-It Imager Benchtop UV Transilluminator) 6× DNA gel loading dye (NEB, B7021S) QIAquick Gel extraction kit (Qiagen, 28704) Thermocycler Agarose gel electrophoresis equipment.
Article Title: Nucleotide resolution sequencing of N4-acetylcytidine in RNA
Article Snippet: Materials TGIRT-III reverse transcription enzyme (INGEX) 5× TGIRT reaction buffer (user prepared with nuclease-free reagents, 2.25M NaCl, 100mM Tris-HCL pH7.5) RNasin plus, 40units/μL (Promega, N2611) Optimized dNTP mix [10mM dTTP, 10mM dCTP, 10mM dATP, 5mM dGTP] (prepared from 100mM dNTPs set, NEB N0446S) Phusion High-Fidelity PCR Kit (New England Biolabs, E0553S) Agarose LE, Molecular Biology Grade (Thomas Scientific, C996H59) 100bp DNA Ladder (New England Biolabs, N3231S) SYBR Safe DNA Gel Stain (Invitrogen, {"type":"entrez-protein","attrs":{"text":"S33102","term_id":"420481","term_text":"pir||S33102"}} S33102 ) UltraPure TBE Buffer, 10× (Invitrogen, 15581044) UV transilluminator (UVP MultiDoc-It Imager Benchtop UV Transilluminator) 6× DNA gel loading dye (NEB, B7021S) QIAquick Gel extraction kit (Qiagen, 28704) Thermocycler Agarose gel electrophoresis equipment.
Agarose Gel Electrophoresis:Article Title: Nucleotide resolution sequencing of N4-acetylcytidine in RNA
Article Snippet: TGIRT-III reverse transcription enzyme (INGEX) 5× TGIRT reaction buffer (user prepared with nuclease-free reagents, 2.25M NaCl, 100mM Tris-HCL pH7.5) RNasin plus, 40units/μL (Promega, N2611) Optimized dNTP mix [10mM dTTP, 10mM dCTP, 10mM dATP, 5mM dGTP] (prepared from 100mM dNTPs set, NEB N0446S) Phusion High-Fidelity PCR Kit (New England Biolabs, E0553S) Agarose LE, Molecular Biology Grade (Thomas Scientific, C996H59) 100bp DNA Ladder (New England Biolabs, N3231S) SYBR Safe DNA Gel Stain (Invitrogen, {"type":"entrez-protein","attrs":{"text":"S33102","term_id":"420481","term_text":"pir||S33102"}} S33102 ) UltraPure TBE Buffer, 10× (Invitrogen, 15581044) UV transilluminator (UVP MultiDoc-It Imager Benchtop UV Transilluminator) 6× DNA gel loading dye (NEB, B7021S) QIAquick Gel extraction kit (Qiagen, 28704) Thermocycler Agarose gel electrophoresis equipment.
Article Title: Nucleotide resolution sequencing of N4-acetylcytidine in RNA
Article Snippet: Materials TGIRT-III reverse transcription enzyme (INGEX) 5× TGIRT reaction buffer (user prepared with nuclease-free reagents, 2.25M NaCl, 100mM Tris-HCL pH7.5) RNasin plus, 40units/μL (Promega, N2611) Optimized dNTP mix [10mM dTTP, 10mM dCTP, 10mM dATP, 5mM dGTP] (prepared from 100mM dNTPs set, NEB N0446S) Phusion High-Fidelity PCR Kit (New England Biolabs, E0553S) Agarose LE, Molecular Biology Grade (Thomas Scientific, C996H59) 100bp DNA Ladder (New England Biolabs, N3231S) SYBR Safe DNA Gel Stain (Invitrogen, {"type":"entrez-protein","attrs":{"text":"S33102","term_id":"420481","term_text":"pir||S33102"}} S33102 ) UltraPure TBE Buffer, 10× (Invitrogen, 15581044) UV transilluminator (UVP MultiDoc-It Imager Benchtop UV Transilluminator) 6× DNA gel loading dye (NEB, B7021S) QIAquick Gel extraction kit (Qiagen, 28704) Thermocycler Agarose gel electrophoresis equipment.
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